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Lab Rats

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Reverse Transcription PCR [Jul. 26th, 2011|11:51 pm]
Lab Rats


Why does my RT-PCR suck? Because the RT-extension primer has a nasty hairpin Tm one degree below the protocol's RNA-to-DNA temp. Why didn't I know this? I assumed the previous worker checked the primer design before placing the order. Why didn't using the alternate primers help? Because they were designed by a second person who hadn't checked their design for secondary structures. What's the lesson? Don't assume anything's been checked unless the analysis is written down.

The good news is, now I know why my experiments were broken, and I'm getting up to speed on primer design damn fast.

[User Picture]From: broken010408
2011-07-28 01:49 am (UTC)

The First Rule Of Lab Existence

" if it isn't written down, signed, dated, and reviewed in a timely manner It Didn't Happen" according to my first and beloved supervisor J (repeatedly- guess its a FDA thing )
biotech drove her back to teaching btw
biotech drove most of us to other careers in fact- lots of nurses, teachers,CNAs...
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