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Lab Rats

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Protocol Hunt [Feb. 13th, 2012|12:37 pm]
Lab Rats

lab_gripes

[benndragon]
[mood |determined]

For various reasons, I find myself without my own lab protocols (I'm not currently working in a lab, much as I wish I was). I was hoping folks here would either be willing/able to share their protocols or have online resources they like to use. I'm looking particularly for cell culture, transformation/infection, cloning, immunohistochemistry, and cellular assay (e.g. ELISA) protocols, but certainly wouldn't mind protocols for extraction, purification, and analysis of DNA, RNA, and proteins as well. All help is very much appreciated!
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Comments:
[User Picture]From: viva_la_vanille
2012-02-14 11:52 am (UTC)
There are a set of books called Current Protocols in _____ that I have found pretty useful as a resource. My lab has a hard copy of Current Protocols in Molecular Biology, and I like to flip through it when I have free time. They have many basic techniques for each field they cover, and offer an introduction/explanation as well as the protocol and troubleshooting tips. They're a pretty easy read and frequently updated.

There are also Cold Spring Harbor Protocols, published by that institution...

Both of these are available online, and hopefully at your local university library.

Best of luck!
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[User Picture]From: ignis
2012-02-16 12:45 pm (UTC)
That ^^^^
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