March 17th, 2007

GFP

(no subject)

Please help me!!! I think I killed a $4,000 FPLC column by forgetting about it and it ran dry!!!!

It is a sephadex 75 prep column, about 320 ml volume and it was pumped dry about 10 hours prior to me finding it. I have it running at 1 ml/ min overnight ( i have a 2 L beaker filled with ultrapure water to hydrate it. I don't know what else to do. I'll come in tomorrow to refill the beaker and to keep it running. I figure I will reverse the column on monday and run it upside down to see if that will also help remove air.

any and all suggestions welcomed. Boss gets back from out of town on weds.