Over recent summer holidays my lab received an inducible expression system kit which I ordered before I went off to enjoy the sunshine. Unfortunately, who ever recieved it stored it in the normal freezer ... which would of been fine if there wasn't a GLYCEROL stock of the host strain required for transformations! So of course, by the time I went to thaw it out they were DEAD, and now I have to order new ones, which have to come in from o/s... so now I have to waste my time waiting for them to come in. Argh! Not Happy Jan!