June 11th, 2007

E. Coli Culture Q

So my lab wants to culture e. coli by throwing some from a plate into LB, shaking overnight, then transplanting some of that media into a fresh vial. This val-to-vial process would continue for however long we need them. However, I've only done the plate--> vial process for PCR purposes so I have no idea what the concentration should be. Has anyone done this before who could give me a per ml LB concentration? Feel free to ask clarifying questions because I'm pretty sure I'm laying this out in a retarded fashion. Thanks1
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