August 12th, 2007

help!

So I'm trying to express a seed storage protein gene for the second half of my dissertation. I have it in the vector and now am at the stage of putting it into the Rosetta-gami cells. I've done heat shock transformations before and been sucessful with them - they really aren't that hard - however, this bug doesn't seem to like me. I used the protocol that was in the Novagen Cell manual and let it grow for over 24 hours. I don't see any colonies. Anyone work with these before and could offer advice on growing them up or how long it takes for these things to grow? Thanks.
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