Measure 600mL H2O to a 1L graduated cylinder, begin stirring.
Using current cylinder to measure, add 350mL MeOH to the cylinder and stir for 30 min.
Using small cylinder to measure, add 45mL TEA to the cylinder and stir for 30 min.
Using volumentric pipette, add 5mL HFIP to cylinder and stir 60 min.
Filter and sonicate.
We regularly see the main peak elute somewhere between 17 and 22 minutes, but we've never seen 15 or 25. We keep telling him that oligos are extremely sensitive to organic solvents and ion pair reagents, but he doesn't seem to get it. He keeps thinking that everything should be as predictable as small molecules. This isn't a small molecule. It's not going to act like one. Get used to it.
And I don't know how he made it 16 years in industry without learning that you don't measure organics directly into water. He didn't mention using a hood or covering the cylinder. I hope he's doing both, but it's possible that he's doing neither.