silly little crustacean girl (ravenword) wrote in lab_gripes,
silly little crustacean girl


Does anyone know of a good anti beta-galactosidase antibody that works well for immunofluorescence? I know what you're thinking, use Xgal. But we're trying to find something that will detect the enzyme even after we've fixed the crap out of our tissue with paraformaldehyde.

I looked up some papers and found MANY Materials and Methods sections describing antibodies that have been discontinued or that were made by a company that doesn't exist anymore (even when restricting my search to the last two years or so), but found nothing currently useful. I wrote to the tech support people at the company that acquired one of the now-defunct companies and they recommended one of their products (AbD Serotec AHP1292), but I can't find any papers that have used it. Also contacted the PI of a lab that gave us the idea to find this antibody originally (his protocol refers to one that no longer exists) and haven't heard back.


  • Grad school hangups - poll!

    Hi all: I'm polling scientists (mostly grad students) for personal experience -- any comments or insight on the following would be great.…

  • OMG

    The title of this post just about sums up everything that I heard yesterday: I went to Maastricht at the request of the committee investigating the…

  • Help me lab gripes, you're my only hope

    So I'm doing long-term fluorescent studies on Hela cells. Well, I used to do them in another lab, ever since we moved nothing is working anymore.…

  • Post a new comment


    default userpic

    Your IP address will be recorded 

    When you submit the form an invisible reCAPTCHA check will be performed.
    You must follow the Privacy Policy and Google Terms of use.