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ELISAs [Nov. 1st, 2008|10:55 pm]
Lab Rats

lab_gripes

[bubbleteagirl02]
So, first off, 'ello all!

Second, if anyone works with mice, perhaps you can help me with this question.

Do you know of any company (not R&D or PBL) that makes a good Interferon-alpha ELISA for mouse serum that is really sensitive? I just ran the R&D ELISA, know I did everything perfectly, and it stunk, royally. Thus I am frustrated at the lack of good ELISA readings (the standard curve wasn't even graphable!) and wondering if there is another good ELISA out there or if I need to start looking for other readouts for Interferon-alpha.
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Comments:
[User Picture]From: bubbleteagirl02
2008-11-02 03:51 am (UTC)
Oh, and the last ELISA I did, I had a beautiful standard curve even though the rest of the ELISA was less than perfect (the hazards of poor sample labeling).

And just as a heads up, I may have just graduated, but I did over a year of research in college in addition to all our very hands on labs where some of our work went towards helping professor's projects. No offense but I am pretty good in lab, not perfect, but I typically can get standard curves to work.
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[User Picture]From: ignis
2008-11-02 04:11 am (UTC)
Like I said, I didn't mean to criticize you. I've found research to be very zen like. When you just start out, you know you know nothing... then after a couple years, you think you know what you are doing.... then after several more years, you realize that while you know a lot, you know nothing. I spent 5 years in a lab before starting medical school and I trained many people. I got a kick out of some people's frustrations at how an experiment works one week, but not the next, and then magically started working again. It's funny how scientific and rational people can become so superstitious. The MDs had the hardest time with the rhythm of science, some times stuff just doesn't work and it's only after you prove that it doesn't work... does it really not work! Often it's because they were rushed in the lab, or their technique was off because they were so 'perfect' that they over washed something. Anyways, bed time.
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[User Picture]From: bubbleteagirl02
2008-11-02 04:15 am (UTC)
LOL! Thankfully I have a low stress environment in lab, well at least when we are not rushing to get downstairs to inject the mice before the lights turn off. It was just annoying because I was very careful to pipet everything correctly and mix everything as the instructions said, even using a plate washer this time instead of a wash bottle like the direction's said and it still fails.

Anyways, thank you very much for you input! Its actually helped more than you think because I had to go back and think about exactly what I did. Still don't know why the ELISA didn't work.
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