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ELISAs [Nov. 1st, 2008|10:55 pm]
Lab Rats

lab_gripes

[bubbleteagirl02]
So, first off, 'ello all!

Second, if anyone works with mice, perhaps you can help me with this question.

Do you know of any company (not R&D or PBL) that makes a good Interferon-alpha ELISA for mouse serum that is really sensitive? I just ran the R&D ELISA, know I did everything perfectly, and it stunk, royally. Thus I am frustrated at the lack of good ELISA readings (the standard curve wasn't even graphable!) and wondering if there is another good ELISA out there or if I need to start looking for other readouts for Interferon-alpha.
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[User Picture]From: bubbleteagirl02
2008-11-02 04:15 am (UTC)
LOL! Thankfully I have a low stress environment in lab, well at least when we are not rushing to get downstairs to inject the mice before the lights turn off. It was just annoying because I was very careful to pipet everything correctly and mix everything as the instructions said, even using a plate washer this time instead of a wash bottle like the direction's said and it still fails.

Anyways, thank you very much for you input! Its actually helped more than you think because I had to go back and think about exactly what I did. Still don't know why the ELISA didn't work.
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